Journal: Cell

Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading

doi: 10.1016/j.cell.2019.04.037

Figure Lengend Snippet: Key Resources Table

Article Snippet: The dishes were then coated with 10 μg/ml human plasma fibronectin (Merck Millipore) at 37°C for 1 hour and blocked with 1% w/v BSA for additional 30 mins and subsequently washed thrice with 1XPBS and once with phenol free incomplete media.

Techniques: Purification, Transduction, Recombinant, Clinical Proteomics, Avidin-Biotin Assay, Membrane, Transfection, Plasmid Preparation, Stable Transfection, Expressing, Mutagenesis, Derivative Assay, Control, Construct, shRNA, Software

Journal: APL Bioengineering

Article Title: Extracellular matrix alignment dictates the organization of focal adhesions and directs uniaxial cell migration

doi: 10.1063/1.5052239

Figure Lengend Snippet: Microcontact-printed ECM screening arrays. (a) Photomask containing 252 individual ECM conditions, each containing a unique randomly generated array of line elements with varying alignments (angular dispersion), line densities, and line widths. (b) Overview of the negative microcontact-printing procedure. Briefly, AlexaFluor555-conjugated fibronectin (Fn555, red) was uniformly coated on flat PDMS stamps and a UV-ozone activated stamp containing the negative of the intended patterns was applied to remove background areas before transferring the remaining areas to the eventual cell culture substrate. (c) Tile-scan fluorescence image of a subsection of the Fn-printed (red) ECM screening array seeded with phalloidin-AlexaFluor488 stained 3T3s (green) (scale bar: 1 mm). (d) Fn555 microcontact-printed patterns demonstrating control over line alignment (left), line density (middle), and line width (right) (scale bars: 10 μ m).

Article Snippet: To enable pattern visualization, fibronectin from human plasma (Fn, Corning, Corning, NY) was fluorescently tagged with AlexaFluor555 succidinyl ester following the manufacturer's protocol (Invitrogen, Carlsbad, CA).

Techniques: Generated, Dispersion, Transferring, Cell Culture, Fluorescence, Staining, Control